THE RADIOAUTOGRAPHIC DETECTION OF DNA WITH TRITIATED ACTINOMYCIN D
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چکیده
منابع مشابه
The Radioautographic Detection of Dna with Tritiated Actinomycin D
The specificity with which actinomycin D is bound to DNA both in vivo and in test tube experiments (for a review, see Reich and Goldberg, 1964) and the recent availability of the drug in tritiated form with high specific activity have suggested its possible usefulness as a means to detect DNA radioautographically in fixed cytological preparations. Brachet and Ficq (1965) had previously employed...
متن کاملDetection of Dna by Tritiated Actinomycin D on Ultrathin Frozen Sections
Ultrathin frozen sections of fresh liver tissue were floated on actinomycin D-(3)H. Quantitative high resolution radioautography was performed to determine the value of the method for detection of DNA by electron microscopy. A complete series of control experiments involving various treatments of frozen sections with enzymes (pronase, DNase) and 0.1 N HCl were also carried out to determine the ...
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Actinomycin D blocks DNA-dependent RNA synthesis by binding to DNA (Reich et al., reference 15). On a cellular level, several radioautographic studies have demonstrated the in vivo binding sites of radioactive actinomycin (de Vitry, reference 19; Fraccaro et al., reference 3; Goldstein et al., reference 8; Ro et al., reference 16; Rothstein et al., reference 17; Sonneborn and Rothstein, referen...
متن کاملExperimental studies with actinomycin D.
Actinomycin D has been tested on mouse tu mors, with varying degrees of success, as an anti-neoplastic agent. Dramatic inhibition of some ascites (mouse) tumors has been reported by Sugiura (14) and Gregory et al. (6). Reilly (11) and Sugiura (14) are in agreement that actino-mycin D has slight antineoplastic activity against S-180, and Farber (3) demonstrated antitumor activity against the mal...
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Euglena gracilis offers a particularly suitable system for investigating some of the fundamental processes involved in the provision, transmission, and translation of information required for the building of specific cytoplasmic structures. Cells grown in the dark do not have a defined chloroplast structure; they are etiolated cells (I). Light induces profound changes in the morphology and phys...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1967
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.35.3.713